ZAP-70 (Phospho-Tyr315)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed to detect phosphorylated ZAP-70 at tyrosine 315 directly in cultured cells. It uses an indirect ELISA format in which phospho-specific primary antibodies capture ZAP-70 (Phospho-Tyr315) and HRP-conjugated secondary antibodies catalyze a colorimetric reaction read at 450 nm. The assay is lysate-free, high-throughput, and sensitive, allowing researchers to measure relative levels of phosphorylated ZAP-70 in cultured cells and to screen for the effects of various treatments, inhibitors, or activators on ZAP-70 phosphorylation.

Reactivity is reported for Human and Mouse samples, the dynamic range is stated as > 5000 Cells, and the method is specified for Adherent Cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method is provided to determine cell density so absorbance values can be normalized to cell numbers. The target is the Phospho sub-type of ZAP-70, with gene symbol ZAP70, Gene ID 7535, and UniProt ID P43403.