XRCC1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit enables detection and qualitative determination of XRCC1 protein in cultured cells using a colorimetric readout measured at 450 nm. The assay uses an indirect ELISA format in which XRCC1 is captured by target-specific primary antibodies, with HRP-conjugated secondary antibodies catalysing a colour reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to normalise absorbance values to cell density. Because the determination is qualitative, multiple normalisation methods are required.

Designed to be lysate-free and suitable for high-throughput screening, the kit is reactive with human, mouse and rat samples and is supplied in a 96-well microplate format with a dynamic range of > 5000 cells. It is intended primarily for adherent cells, with a Poly-L-Lysine pre-coating option available for suspension and loosely attached cells. The XRCC1 protein targeted by the kit is involved in DNA single-strand break repair and base-excision repair pathways, and the kit falls within Assay Genie's Epigenetics and Nuclear Signaling research area. The plate is read at 450 nm immediately after the addition of stop solution, and the kit is stored at 4 °C for 6 months.