WNK1 (Phospho-Thr58)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the detection of phosphorylated WNK1 at threonine 58 in cell lysates, offering high sensitivity and specificity for accurate and reproducible results across a variety of research applications. WNK1 is a serine/threonine kinase that plays a crucial role in regulating ion transport and maintaining cellular homeostasis, with phosphorylation at Thr58 implicated in physiological processes including blood pressure regulation and cell proliferation. Dysregulation of WNK1 signaling has been associated with conditions such as hypertension and neurological disorders, making it a target of interest for therapeutic research.

The kit provides a convenient, lysate-free, high-throughput workflow using an indirect ELISA format with colorimetric detection at 450 nm and a dynamic range of > 5000 cells. It is reactive with human, mouse, and rat samples and is intended for use with adherent cells. The assay enables measurement of relative amounts of phosphorylated WNK1 in cultured cells and can be used to screen the effects of various treatments, inhibitors, or activators on WNK1 phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control in normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density after the colorimetric measurement.

The kit is provided in a 2 x 96-well microplate format. Database identifiers for the WNK1 target include Gene ID 65125 and UniProt ID Q9H4A3.