WEE1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit is designed to detect WEE1 protein levels in cultured cells. The assay uses a colorimetric detection method at 450 nm in a lysate-free, high-throughput workflow: WEE1 is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies catalyse the colour reaction upon substrate addition.

Reactivity is reported for human, mouse, and rat samples, with a dynamic range of > 5000 cells. Because the cell-based format is qualitative, the kit includes a monoclonal anti-GAPDH antibody to serve as an internal positive control for normalising target absorbance, plus Crystal Violet whole-cell staining to determine cell density and adjust for plating differences. The kit is intended for measuring relative WEE1 levels in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on WEE1. Storage is at 4°C with a stated stability of 6 months. The product falls within the cell cycle research area.

WEE1 (Wee1-like protein kinase) is a nuclear protein kinase of the WEE family that catalyses the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, playing a critical role in the G2/M transition of the mitotic cell cycle and coordinating the switch between DNA replication and mitosis. Dysregulation of WEE1 has been linked to cancer and developmental disorders, making it a relevant target for cell cycle and biomarker research. The indicated sample type is adherent cells.