Vitamin D3 (VD3) ELISA Kit

Designed for research use only, this competitive ELISA kit from Assay Genie enables quantitative measurement of Vitamin D3, also known as cholecalciferol or the "sunshine vitamin", in serum, plasma and other biological fluids. Using a colorimetric competitive ELISA principle, the assay is suited to studies of vitamin D3 biology and its links to bone health, immune function, calcium and phosphorus regulation, and conditions such as osteoporosis, cardiovascular disease and autoimmune disorders.

Manufacturer-reported performance includes a detection range of 1.56-100 ng/mL, a sensitivity of 0.94 ng/mL, a recovery of 80%-120%, and an assay time of 2 h 30 min. The kit recognises universal VD3 in samples, with no significant cross-reactivity or interference between universal VD3 and analogues reported. Optical density is read at 450 nm ± 2 nm, and application areas highlighted by the manufacturer include signal transduction and metabolism research.

Supplied components include a dismountable micro ELISA plate, reference standard, concentrated biotinylated detection antibody, HRP conjugate, sample and assay diluents, wash buffer, substrate reagent, stop solution, plate sealers, a technical manual and a certificate of analysis. The manufacturer also provides detailed preparation guidance for serum, plasma, cell culture supernatants, urine, cerebrospinal fluid, cell lysates, tissue homogenates, tissue lysates and breast milk to support reliable quantification across a range of biological samples.