Vitamin D Receptor (Phospho-Ser208)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed to detect Vitamin D Receptor phosphorylated at serine 208 in cell samples. It is reactive with human samples and suitable for adherent cells, and uses a colorimetric detection method measured at 450 nm through HRP-conjugated secondary antibodies in an indirect ELISA format, providing qualitative determination of phosphorylated Vitamin D Receptor levels.

The assay operates with a dynamic range of > 5000 Cells and is intended for measuring relative amounts of phosphorylated Vitamin D Receptor in cultured cells, as well as for screening the effects of various treatments, inhibitors, or activators on phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and Crystal Violet whole-cell staining may be used to determine cell density. The target corresponds to the VDR gene (Gene ID: 7421; UniProt ID: P11473).

Recommended storage is at 4°C, with a stated shelf life of 6 months.