Vimentin (Phospho-Ser83)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is designed to analyze the phosphorylation status of vimentin at serine 83 in cell lysates. It targets Vimentin (Phospho-Ser83) and is reactive with Human, Mouse, and Rat samples. The assay uses a colorimetric detection method measured at 450 nm, with a dynamic range of > 5000 Cells.

The kit provides a convenient, lysate-free, high-throughput and sensitive assay that detects Vimentin protein phosphorylation and expression profile in cells. It uses an indirect ELISA format, in which Vimentin (Phospho-Ser83) is captured by specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. The bound HRP enzyme then catalyses a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining can be used to determine cell density for further normalization.

Researchers can use this kit to measure the relative amounts of phosphorylated Vimentin in cultured cells, including adherent cells, as well as to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Vimentin phosphorylation. The kit falls within the Immunology research area.