VEGFB Colorimetric Cell-Based ELISA Kit

The Assay Genie Cell-Based ELISA Kit targets vascular endothelial growth factor B (VEGFB) for measurement across human, mouse, and rat samples. The colorimetric assay uses an indirect ELISA format with readout at 450 nm, in which VEGFB-specific primary antibodies are captured and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density for further normalization.

Designed as a lysate-free, high-throughput solution, the kit measures relative VEGFB protein expression in cultured cells and can be used to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on VEGFB. The source describes VEGFB as a growth factor for endothelial cells belonging to the PDGF/VEGF growth factor family, involved in angiogenesis and the development and maintenance of blood vessels, with dysregulation linked to diseases including cancer, cardiovascular ailments, and metabolic disorders.

The kit is reactive with human, mouse, and rat samples, with a dynamic range stated as greater than 5000 Cells and colorimetric detection at 450 nm. Storage is at 4°C with a 6 Months stability period. Sample types include serum, plasma, and cell culture supernatants, with adherent cells noted as the indicated sample type.