VAV3 Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie designed for the accurate detection of VAV3 levels in cell lysates and tissue homogenates. The assay uses a colorimetric detection method at 450 nm in an indirect ELISA format, providing a high-throughput, lysate-free, and sensitive approach for measuring the relative expression of VAV3 in cultured cells. It can also be used to screen the effects of various treatments, inhibitors, or activators on VAV3.

The kit includes a monoclonal GAPDH antibody as an internal positive control for normalizing target absorbance values, along with a Crystal Violet whole-cell staining step to determine cell density and adjust for plating differences. It shows reactivity with human and mouse samples and supports a dynamic range of > 5000 Cells, with recommended storage at 4°C for 6 months. The product is listed for research in cardiovascular areas.

VAV3 is a guanine nucleotide exchange factor (GEF) that activates RhoA, RhoG and, to a lesser extent, Rac1, and plays a role in angiogenesis, integrin-mediated signaling, wound healing, and immune cell adhesion. Dysregulation of VAV3 has been linked to cancer, immune disorders, and neurodegenerative conditions, making it a relevant target for therapeutic development and disease research.