VAV2 (Phospho-Tyr142)Colorimetric Cell-Based ELISA Kit

The Assay Genie cell-based ELISA kit provides a colorimetric, indirect ELISA format for the qualitative detection of VAV2 phosphorylation at tyrosine 142 in cultured cells. Designed as a lysate-free, high-throughput assay, it enables researchers to measure relative levels of phosphorylated VAV2 and to screen treatments, inhibitors (such as siRNA or chemicals), or activators for their effects on VAV2 phosphorylation.

The workflow uses VAV2 (Phospho-Tyr142)-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density and adjust for plating differences. The kit is reactive with human and mouse samples and is intended for adherent cells, with a stated dynamic range exceeding 5000 cells. Detection is read at 450 nm.

This format makes it suitable for studying VAV2-mediated signaling pathways involved in cell migration, adhesion, and cytoskeletal rearrangement, as well as downstream processes implicated in disease progression.