VAV1 (Phospho-Tyr174)Colorimetric Cell-Based ELISA Kit

Assay Genie offers this cell-based ELISA kit for the accurate measurement of VAV1 phosphorylation at tyrosine 174 in cell lysates. Designed with high sensitivity and specificity, the kit enables reliable and reproducible analysis of VAV1 signalling pathway activation. Reactivity has been validated for human, mouse, and rat samples, with a dynamic range of > 5000 cells.

The assay uses an indirect ELISA format, in which VAV1 (Phospho-Tyr174) is captured by phospho-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyse a colourimetric reaction read at 450 nm. It is suitable for adherent cells and allows the relative amounts of phosphorylated VAV1 in cultured cells to be measured, as well as screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on VAV1 phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining can be used to determine cell density.

VAV1 is a key signalling protein involved in T cell activation, cytokine signalling, and cell migration, with phosphorylation at tyrosine 174 playing a crucial role in regulating its activity and function. Dysregulation of VAV1 phosphorylation has been implicated in immune disorders, cancer, and inflammatory diseases.