USF2 Colorimetric Cell-Based ELISA Kit

Assay Genie offers a cell-based ELISA for qualitative measurement of USF2 protein expression directly in cultured cells, eliminating the need for lysate preparation. The assay runs on a 96-Well Microplate format and uses a colorimetric detection method read at 450 nm, where HRP-conjugated secondary antibodies catalyze a visible color change upon substrate addition. The kit shows reactivity with human, mouse, and rat samples and is intended for adherent cells, with a dynamic range of > 5000 Cells.

USF2 (upstream transcription factor 2) is a basic helix-loop-helix transcription factor that regulates gene expression and participates in cellular processes including proliferation, differentiation, and apoptosis. Aberrant USF2 expression has been associated with cancer, diabetes, and cardiovascular disorders, making it a relevant marker for disease research. The kit falls within the Epigenetics and Nuclear Signaling research area and can be used to screen the effects of treatments, siRNA, chemicals, or other activators on USF2 expression in cells.

Two normalization strategies are incorporated into the workflow: a monoclonal anti-GAPDH antibody serves as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density so absorbance values can be adjusted for plating differences. The kit is stored at 4°C with a stated stability of 6 Months. Database references for the target include Gene ID 7392 and UniProt ID Q15853, with a reported molecular weight of 23,988 Da.