Tyrosine Hydroxylase (Phospho-Ser31)Fluorometric Cell-Based ELISA Kit

A fluorometric Cell-Based ELISA Kit from Assay Genie designed to monitor Tyrosine Hydroxylase (Phospho-Ser31) protein phosphorylation and expression profile in cells. The assay is lysate-free, high throughput and sensitive, and uses an indirect ELISA format in which target-specific primary antibodies capture Phospho-Tyrosine Hydroxylase (Ser31). Dye 1-conjugated and Dye 2-conjugated secondary antibodies then bind the Fc region of the primary antibody, enabling fluorometric readout.

The kit is reactive with Human, Mouse and Rat samples and supports a dynamic range of > 5000 Cells. It is suited to measuring relative levels of phosphorylated Tyrosine Hydroxylase in cultured cells and to screening the effects of treatments, inhibitors (e.g., siRNA or chemicals) or activators on Tyrosine Hydroxylase phosphorylation. Two normalization strategies are provided: a monoclonal anti-GAPDH antibody serves as an internal positive control, and an antibody against the non-phosphorylated counterpart of Tyrosine Hydroxylase is included for normalizing the phosphorylated signal.

Plates are read at Ex/Em of 651/667 nm (Dye 1) and 495/521 nm (Dye 2). Storage is at 4°C with a stated stability of 6 Months. Associated target identifiers: NCBI Gene Symbol TH, Gene ID 7054, UniProt ID P07101, OMIM 191290/605407, Unigene Hs.435609/Hs.523414.