Tyrosine Hydroxylase (Phospho-Ser31)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit enables the accurate measurement of tyrosine hydroxylase phosphorylation at serine 31 in cell lysates. It is a convenient, lysate-free, high-throughput, and sensitive assay designed for adherent cells, with reactivity confirmed in Human, Mouse, and Rat samples.

Detection is colorimetric at 450 nm with a dynamic range of > 5000 Cells. The kit uses an indirect ELISA format in which Tyrosine Hydroxylase (Phospho-Ser31) is captured by phospho-specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density for further normalisation.

Tyrosine hydroxylase is the rate-limiting enzyme in catecholamine biosynthesis — including dopamine, norepinephrine, and epinephrine — and its phosphorylation at serine 31 modulates activity, affecting neurotransmitter levels and neuronal signalling. The kit is suited to measuring relative amounts of phosphorylated tyrosine hydroxylase in cultured cells and to screening the effects of treatments, inhibitors (e.g. siRNA or chemicals), or activators on tyrosine hydroxylase phosphorylation, with relevance to research on neurological disorders including Parkinson's disease and addiction.