Tyrosine Hydroxylase (Phospho-Ser19)Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this cell-based ELISA kit provides a colorimetric method for detecting Tyrosine Hydroxylase phosphorylated at serine 19 in cultured cells. The assay targets the phosphorylated form of tyrosine hydroxylase, the rate-limiting enzyme in the synthesis of catecholamines, and is reactive with human, mouse, and rat samples. Detection is achieved through an indirect ELISA format using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction measured at 450 nm.

The in-cell protocol is lysate-free and designed for adherent cell samples. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, while Crystal Violet whole-cell staining may also be used to adjust results for cell density. The approach supports measurement of relative phosphorylation levels in cultured cells and screening of treatments, inhibitors, or activators on tyrosine hydroxylase phosphorylation.

Tyrosine hydroxylase catalyzes the conversion of tyrosine to dopamine and plays a key role in the physiology of adrenergic neurons. Phosphorylation at serine 19 is linked to regulation of enzyme activity, making this kit a useful tool for research into dopamine signalling pathways and related neurological conditions.