Tyrosinase Colorimetric Cell-Based ELISA Kit

Designed for the detection of tyrosinase levels in cell lysates, this cell-based ELISA kit from Assay Genie offers a high-throughput, lysate-free workflow suitable for adherent cell samples. The kit targets tyrosinase (TYR), a copper-containing oxidase that catalyses the rate-limiting steps in melanin biosynthesis, with reactivity specific to human samples.

The colorimetric detection method uses HRP-conjugated secondary antibodies and is read at 450 nm, with a dynamic range of > 5000 cells. The assay follows an indirect ELISA format in which tyrosinase-specific primary antibodies capture the target protein. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalisation, and Crystal Violet whole-cell staining can be used to determine cell density.

Storage is at 4°C with a stated stability of 6 months. The kit is intended for research applications in metabolism, including studies of skin pigmentation, melanoma, and related skin conditions, and is supplied in a microplate format with associated reagents for the full staining and detection workflow.