TUSC3 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA Kit is designed for the qualitative detection of TUSC3 protein expression in cultured cells. It uses an indirect ELISA format in which TUSC3-specific primary antibodies are captured on the target and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, producing a colorimetric signal upon substrate addition. The kit is described as lysate-free, high-throughput, and sensitive, and can be used to measure relative TUSC3 amounts in cells or to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on TUSC3 levels.

The assay shows reactivity with human, mouse, and rat samples, has a dynamic range of > 5000 Cells, and uses colorimetric detection at 450 nm in a 96-Well Microplate format. Storage is at 4°C for 6 months. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, while Crystal Violet whole-cell staining is used to determine cell density so that absorbance values can be further normalized to cell amounts. The source notes the kit offers high sensitivity and specificity for detection of TUSC3 in cell lysates and supernatants.

TUSC3 (Tumor suppressor candidate 3) is a magnesium transporter that may be involved in N-glycosylation through its association with N-oligosaccharyl transferase, making it a relevant target for research in areas such as cancer, diabetes, and neurodegenerative diseases. According to UniProt and NCBI data cited in the source, the protein has a molecular weight of 40kDa, maps to chromosomal location 8p22 in humans, and is identified by UniProt ID Q13454 and NCBI Gene ID 7991.