TTC23 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA kit is designed to measure TTC23 protein levels in cell samples using a colorimetric readout at 450 nm. The assay uses an indirect ELISA format in which TTC23 is captured by target-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. It is described as a lysate-free, high-throughput, and sensitive workflow intended for cultured cells, including the screening of treatments, inhibitors (such as siRNA or chemicals), or activators that affect TTC23 expression.

Qualitative determination of TTC23 concentration is supported by two normalization methods: a monoclonal anti-GAPDH antibody supplied as an internal positive control, and an optional Crystal Violet whole-cell staining step to account for cell density. The kit is configured for use with adherent cells and human samples, with a stated dynamic range of > 5000 Cells and a 96-Well Microplate format stored at 4 °C for 6 months. Associated target information includes the gene TTC23 (Gene ID: 64927; UniProt ID: Q5W5X9; chromosomal location 15q26.3) and the protein Tetratricopeptide repeat protein 23, also known as HCC-8, with a reported molecular weight of 44,238 Da.