TSC22D1 Colorimetric Cell-Based ELISA

From Assay Genie, the TSC22D1 Colorimetric Cell-Based ELISA is designed for the accurate detection of TSC22D1 levels in cell lysates and tissue homogenates. This Cell-Based ELISA kit is described as offering high sensitivity and specificity, in a convenient, lysate-free, high-throughput format for measuring the relative amounts of TSC22D1 in cultured cells, as well as for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on TSC22D1.

Qualitative determination of TSC22D1 concentration is achieved via an indirect ELISA format: TSC22D1 is captured by TSC22D1-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. The conjugated HRP enzyme catalyses a colourimetric reaction upon substrate addition, measured at 450 nm. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density for further normalisation.

The kit is reactive with Human, Mouse and Rat samples, with a dynamic range of > 5000 Cells, and is supplied in a 96-Well Microplate format with storage at 4°C for 6 Months. It is listed for research use in the Epigenetics and Nuclear Signaling area.