Tryptophan Hydroxylase Colorimetric Cell-Based ELISA Kit

Designed for neuroscience research, this Assay Genie kit provides qualitative detection of Tryptophan Hydroxylase protein in cultured adherent cells using a colorimetric cell-based ELISA method. The workflow uses an indirect ELISA format in which target-specific primary antibodies capture Tryptophan Hydroxylase, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction at 450 nm upon substrate addition. The procedure is lysate-free and high throughput.

Researchers can use the assay to determine relative Tryptophan Hydroxylase amounts in cultured cells or to screen the effects of treatments, inhibitors such as siRNA or chemicals, and activators. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, while the Crystal Violet whole-cell staining method may be used to determine cell density and correct for plating differences.

Tryptophan hydroxylase is the rate-limiting enzyme in serotonin biosynthesis, catalyzing the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan. The kit is reactive with Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells, run in a 96-Well Microplate format. Storage stability is 4 °C for 6 Months.