Trypsin-Citrate Solution (0.25%), 100mL

A sterile enzymatic dissociation solution formulated for routine subculturing of adherent mammalian cells. The 0.25% trypsin blended with citrate chelator gently breaks down adhesion proteins and calcium-dependent junctions between cells and the culture substrate, enabling clean detachment without excessive clumping. Supplied as a 100 mL bottle and applied in standard cell culture workflows including passaging of human pluripotent stem cells, mesenchymal stromal cells, and continuous cell lines. STEMCELL Technologies manufactures this ready-to-use solution to support reproducible cell harvest in research laboratories. Store frozen at -20 degrees C to preserve enzymatic activity, and avoid repeated freeze-thaw cycles by aliquoting into single-use working volumes. Each lot is quality tested for cell-dissociation performance across multiple cell types. The citrate component reduces the need for separate EDTA washing steps, streamlining the dissociation workflow while preserving surface marker integrity for downstream flow cytometry or immunostaining applications.