Collagenase C, ACF, 100 mg

Manufactured by STEMCELL Technologies, this Animal Component Free (ACF) Collagenase C enzyme preparation is supplied as a 100 mg lyophilized vial tailored for research-grade tissue dissociation workflows. The formulation is optimized to digest collagen-rich extracellular matrix during primary tissue isolation, supporting reproducible recovery of cells from organs such as pancreas, liver, and lung under controlled laboratory conditions.

The ACF manufacturing designation means no animal-derived raw materials are introduced during enzyme production, reducing xeno-contamination risk for downstream human cell culture, characterization, and in vitro analysis. Investigators working on pancreatic islet isolation, hepatic cell separation, and similar organ-specific harvesting protocols commonly select this preparation because of its lot-to-lot consistency and defined activity profile. Researchers typically reconstitute the lyophilized powder in a balanced salt solution or suitable buffer immediately before use, then titrate working concentration to match their specific tissue source, density, and digestion time requirements.

For handling, store the unopened vial at -20°C in a sealed, dry container to preserve enzymatic activity across multiple experimental sessions; once reconstituted, aliquot the working solution and freeze for long-term storage, limiting freeze-thaw cycles to protect potency. Each lot is quality tested under STEMCELL Technologies enzyme release specifications to confirm reproducible performance, making this preparation a practical choice for cell isolation studies in diabetes research, regenerative medicine workflows, and other biomedical investigations.