Collagenase A, ACF, 100 mg

STEMCELL Technologies Collagenase A (ACF) is a crude collagenase enzyme preparation manufactured under animal component-free conditions, supplied as a 100 mg vial of lyophilized powder. Derived from Clostridium histolyticum, this enzyme cocktail contains collagenase alongside supplementary proteases used to digest connective tissue during cell isolation workflows. Researchers routinely apply it to dissociate tissues such as liver, lung, heart, adipose, and tumor biopsies into single-cell suspensions for downstream assays including flow cytometry and primary cell culture.

The ACF designation confirms the enzyme is produced without animal-derived raw materials, an important consideration for workflows requiring defined manufacturing conditions or for cells destined for downstream therapeutic research. Each lot is quality tested, and the Certificate of Analysis reports the specific activity in collagenase units per mg. Reconstitute the lyophilized powder in a balanced salt buffer such as HBSS, typically at 1-10 mg/mL depending on tissue density, then combine with complementary enzymes such as dispase, trypsin, or DNase I as dictated by the extracellular matrix composition.

For storage, keep the sealed vial refrigerated and protect from light until first use. After reconstitution, dispense into single-use aliquots and freeze at -20 degrees C to preserve enzymatic activity across multiple experiments. Avoid repeated freeze-thaw cycles. The format ships as a single 100 mg vial intended for research use only, not for diagnostic or therapeutic procedures.