Collagenase Type I, 5 g

Researchers isolating primary cells from solid tissues rely on STEMCELL Technologies Collagenase Type I as a multi-enzyme dissociation reagent. Sourced from Clostridium histolyticum fermentation, each 5 g vial of this lyophilized powder contains collagenase along with neutral protease, caseinase, clostripain, and trypsin-like activities that together degrade the interstitial extracellular matrix of connective tissue while preserving cell-surface markers and viability.

Working concentrations typically range from 0.5 to 2 mg/mL in HBSS or a comparable balanced salt buffer, with digestions performed at 37 °C for 20 to 45 minutes under gentle agitation. The crude enzyme profile is particularly effective for harvesting pancreatic islets, hepatocytes, adipocytes, and lung or intestinal epithelial organoids, where maintaining cell-cell contacts and stromal architecture matters as much as releasing individual cells. Supplementation with DNase I at 10 to 100 µg/mL reduces clumping from released genomic DNA during longer incubations.

For storage, the lyophilized powder remains stable at 2 to 8 °C when kept dry, while reconstituted stocks should be aliquoted and stored at -20 °C to limit activity loss from repeated freeze-thaw cycles. The 5 g pack size suits laboratories running rodent or human tissue dissociation in moderate-to-high throughput, offering cost-per-digestion savings over single-experiment vials. Each lot ships with a certificate of analysis detailing collagen digestion unit activity, endotoxin burden, and mycoplasma screening, supporting downstream applications in cell therapy, organoid culture, and regenerative medicine research.