Collagenase B, ACF, 100 mg

Tissue dissociation workflows in primary cell isolation rely on a Class II collagenase blend from STEMCELL Technologies. Collagenase B (ACF, 100 mg) is an Animal Component-Free preparation that digests extracellular connective matrix without exposing downstream cultures to animal-derived raw materials. The lyophilized enzyme, purified from Clostridium histolyticum, is supplied in a single vial and is commonly selected for adipose, lung, and stromal tissue digests. Each 100 mg aliquot supports multiple reactions and integrates into cell culture protocols for releasing fibroblasts, adipocyte stromal cells, and hepatocytes from intact tissue. Store the powder at -20°C protected from light, and reconstitute only the working volume needed; avoid repeated freeze-thaw cycles to preserve activity. The ACF manufacturing process reduces the risk of adventitious agents, supporting regulated research, regenerative medicine, and xeno-free culture systems. Reconstituted enzyme is typically diluted in balanced salt solution to working concentrations of 0.1 to 2 mg/mL, depending on tissue density and incubation time.