Collagenase Type I, 100 mg

Supplied as a 100 mg lyophilized vial, this Collagenase Type I enzyme preparation from STEMCELL Technologies is derived from Clostridium histolyticum and formulated for enzymatic dissociation of connective tissue in primary cell isolation workflows. The blend delivers collagenase as the principal activity, complemented by clostripain, trypsin-like, and caseinase activities that together degrade the collagen-rich extracellular matrix surrounding adherent cells. Researchers apply this reagent to liberate pancreatic islets from rodent or human donor pancreas for transplant and diabetes research, to isolate hepatocytes from liver tissue, and to release adipocytes from stromal fractions for metabolic studies. Each vial is reconstituted in a buffered salt solution or culture medium to a working stock, with typical final assay concentrations ranging from 0.5 to 2.5 mg/mL depending on tissue density and species. The lyophilized format supports refrigerated storage and reconstitution on the day of use, minimizing freeze-thaw loss of enzymatic activity. STEMCELL Technologies packages this enzyme as a research-grade single-use reagent suitable for downstream cell culture applications, including islet transplantation studies, regenerative medicine workflows, and in vitro tissue digestion protocols.