Trk B (Phospho-Tyr705)Colorimetric Cell-Based ELISA Kit

From Assay Genie comes a cell-based ELISA kit designed for the qualitative detection of Trk B phosphorylated at tyrosine 705. The colorimetric assay runs at 450 nm and is configured as an indirect ELISA, in which Trk B (Phospho-Tyr705)-specific primary antibodies capture the target and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition.

The assay is lysate-free and works on adherent cells, enabling high-throughput measurement of relative Trk B phosphorylation levels in cultured cells. It can be used to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on Trk B phosphorylation. Reactivity is reported for human, mouse, and rat samples. Because the assay is qualitative, normalization is performed using two approaches: a monoclonal anti-GAPDH antibody supplied as an internal positive control, and Crystal Violet whole-cell staining to determine cell density and adjust for plating differences.

The kit targets Trk B, the product of the NTRK2 gene and a member of the neurotrophic tyrosine receptor kinase (NTRK) family. Trk B is a receptor for brain-derived neurotrophic factor (BDNF) as well as neurotrophin-3, -4 and -5. The dynamic range is stated as greater than 5000 cells.