Trk A (Phospho-Tyr701)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed to measure phosphorylated Trk A (Tyr701) levels in cell lysates and tissue samples using a Colorimetric detection method. The assay provides a convenient, lysate-free, high-throughput and sensitive format for detecting Trk A protein phosphorylation and expression profiles in cells, supporting measurement of relative phosphorylation in cultured cells and screening of the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on Trk A phosphorylation.

The kit uses an indirect ELISA format: Trk A (Phospho-Tyr701)-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody and catalyze a colorimetric reaction upon substrate addition, measured at 450 nm. Reactivity is established for Human, Mouse, and Rat samples, with a dynamic range of "> 5000 Cells." A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method may be applied to determine cell density so that absorbance values can be normalised to cell amounts. The assay is intended for use with adherent cells.

TrkA is a receptor tyrosine kinase of the Trk family and acts as a high-affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5, but not brain-derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1, and the receptor has a crucial role in the development and function of the nociceptive reception system. The target is encoded by NTRK1 (Gene ID: 4914; UniProt ID: P04629).