Trk A Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed for the qualitative detection of Trk A protein expression in cultured cells using a colorimetric readout at 450 nm. The lysate-free, indirect ELISA format uses Trk A-specific primary antibodies together with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. The assay is suitable for measuring relative Trk A levels in cultured cells and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on Trk A.

Reactivity is reported for human, mouse, and rat samples, with a stated dynamic range of more than 5,000 cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and following the colorimetric HRP measurement a Crystal Violet whole-cell staining method can be used to determine cell density, allowing absorbance values to be normalized to cell amounts and plating differences to be adjusted. The kit is supplied in a 96-well microplate format, stored at 4 °C with a stability of 6 months, and is intended for use with adherent cells. Trk A, encoded by the gene symbol NTRK1, is a receptor tyrosine kinase that acts as a high-affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5.