TRIP13 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA kit is designed for the sensitive and specific detection of TRIP13 levels in cell lysates and tissue homogenates. The assay uses a colorimetric detection method read at 450 nm and is reactive with human, mouse, and rat samples. With a dynamic range of > 5000 Cells, the kit offers a convenient, lysate-free, high-throughput format suitable for adherent cell samples.

TRIP13 is a key protein involved in cell division and DNA repair processes, making it a vital target for cancer research and drug development. The kit employs an indirect ELISA format in which TRIP13-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values. Following the colorimetric measurement of HRP activity, the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalized to cell amounts and plating differences to be adjusted.

The kit can be used both for measuring the relative amounts of TRIP13 in cultured cells and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on TRIP13. Storage stability is rated at 4°C for 6 Months.