TRADD Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the measurement of TRADD (tumor necrosis factor receptor type 1-associated DEATH domain protein) levels in cell lysates, tissue homogenates, and cell culture supernatants. The kit targets TRADD, a key signalling molecule involved in regulating cell death and survival pathways, and is intended for research into diseases such as cancer, inflammation, and autoimmune disorders.

Reactive with human and mouse samples, the assay uses a colorimetric detection method at 450 nm on a 96-well microplate, with a dynamic range of > 5000 Cells. Adherent cells are a supported sample type. The indirect ELISA format captures TRADD with TRADD-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP enzyme to catalyse a colour reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalising target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be normalised to cell amounts.

Storage is at 4°C with a stability of 6 Months. The stated research area is cell death.