TRA-2 alpha Colorimetric Cell-Based ELISA

This cell-based ELISA from Assay Genie provides qualitative detection of TRA-2 alpha protein expression in cultured adherent cells using an indirect colorimetric format read at 450 nm. The assay is lysate-free and supports high-throughput screening of treatments, inhibitors such as siRNA or chemicals, and activators. Reported reactivity covers human and mouse, with a stated dynamic range of > 5000 cells and storage at 4°C for 6 months in a 96-well microplate format.

The assay uses primary antibodies specific for TRA-2 alpha with HRP-conjugated secondary antibodies that bind the Fc region of the primary antibody. A monoclonal anti-GAPDH antibody serves as an internal positive control for normalizing absorbance values, and Crystal Violet solution enables whole-cell staining to adjust for plating variation. The target gene is TRA2A (Gene ID 29896, UniProt Q13595), a sequence-specific RNA-binding protein of the SR splicing factor family that participates in pre-mRNA splicing regulation, categorized under Epigenetics and Nuclear Signaling research.