TOP2A Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the precise measurement of TOP2A (DNA topoisomerase 2-alpha) levels in cell lysates and tissue samples. It uses an indirect ELISA format in which TOP2A is captured by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as serve as an internal positive control, and Crystal Violet whole-cell staining may be used to determine cell density for normalization of the qualitative readout.

TOP2A is described as a key enzyme involved in DNA replication and cell division, and abnormal expression has been linked to various types of cancer, making it a vital target for studying cancer development and progression. The assay is described as convenient, lysate-free, high throughput, and sensitive, and can be used for measuring the relative amounts of TOP2A in cultured cells as well as screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators. Reactivity is for human samples, with the procedure suitable for adherent cells.

The kit is supplied in a 96-Well Microplate format with a stated dynamic range of > 5000 Cells, colorimetric detection at 450 nm, and storage stability of 4°C for 6 months. Supplied components include a 100x Anti-Phospho Target Antibody, 100x Anti-Target Antibody, Anti-GAPDH Antibody, and HRP-Conjugated Anti-Rabbit and Anti-Mouse IgG antibodies, along with buffers, substrate, stop solution, and Crystal Violet Solution. Associated database identifiers for the TOP2A target include Gene ID 7153 and UniProt ID P11388, with a molecular weight of 182,681 Da.