TNNI3 (Phospho-Ser43)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA from Assay Genie is designed to detect phosphorylation at serine 43 of troponin I type 3 (TNNI3) in cell lysates and tissue samples. The assay is reactive with Human, Mouse, and Rat samples, uses a colorimetric detection method measured at 450 nm, and supports a dynamic range of more than 5,000 cells. The qualitative, indirect ELISA format captures TNNI3 (Phospho-Ser43) with phospho-specific primary antibodies, while HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition.

Phosphorylation of serine 43 on troponin I type 3 plays a vital role in cardiac muscle contraction and relaxation, making it a key marker in the study of cardiovascular diseases and heart failure. The lysate-free, high-throughput assay can be used to measure relative levels of phosphorylated TNNI3 in cultured cells and to screen for the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on TNNI3 phosphorylation.

An anti-GAPDH monoclonal antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density, allowing absorbance values to be normalized to cell amounts. Associated identifiers include Gene ID 7137 and UniProt ID P19429.