TNF12 Colorimetric Cell-Based ELISA

Assay Genie's TNF12 Colorimetric Cell-Based ELISA enables quantitative detection of TNF-alpha cytokine directly within adherent cell monolayers, eliminating traditional lysate preparation steps. The in-cell ELISA workflow begins with cell culture in the provided microplate format, followed by stimulation, fixation, permeabilization, and primary antibody incubation against TNF-α protein. Colorimetric readout occurs on a standard plate reader, producing absorbance values proportional to intracellular TNF-α levels with 100 μL sample volume per well. This approach is valuable for inflammation research, evaluating cytokine induction in macrophage or monocyte cell lines such as THP-1 or RAW264.7 following LPS or pharmacological treatment. Each kit contains sufficient reagents for 96 Assays and should be stored refrigerated to preserve antibody and substrate stability through the kit shelf life. Researchers use this in vitro assay format to screen anti-inflammatory compounds, characterize signaling pathway activation, and validate knockdown efficiency at the protein level without Western blot workflows.