TMPRSS3 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA kit provides a high throughput, lysate-free workflow for detecting and quantifying TMPRSS3 levels in cultured cells. It uses an indirect ELISA format in which TMPRSS3-specific primary antibodies are bound by HRP-conjugated secondary antibodies, generating a colourimetric signal measured at 450 nm. The kit is reactive with human and mouse samples and supports a dynamic range of > 5000 Cells.

Supplied in a microplate format, the assay includes an anti-GAPDH monoclonal antibody to serve as an internal positive control for normalising target absorbance values, alongside a Crystal Violet whole-cell staining method that accounts for plating differences between wells. Recommended storage is at 4 °C with a stated stability of 6 months.

TMPRSS3 (Transmembrane protease serine 3) is a probable serine protease of the peptidase S1 family, mapped to chromosomal location 21q22.3 (Gene ID: 64699; UniProt ID: P57727). It acts as a permissive factor for cochlear hair cell survival and activation at the onset of hearing, and defects in TMPRSS3 are the cause of deafness autosomal recessive types DFNB8 and DFNB10. The kit is intended for qualitative determination of TMPRSS3 expression profiles and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on the target.