TISB Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the detection and measurement of TISB (described by the manufacturer as a Tumor-Induced Senescence Biomarker, corresponding to gene symbol ZFP36L1) in adherent cell samples, supporting research in cancer biology, cell senescence, and drug development. The assay uses a colorimetric detection method measured at 450 nm and is formatted for use with a 96-well microplate.

It functions as a lysate-free, high-throughput, sensitive indirect ELISA. TISB-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling a colorimetric reaction upon substrate addition. The kit is reactive with Human, Mouse, and Rat samples, with a stated dynamic range of > 5000 cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control, and Crystal Violet whole-cell staining is provided to determine cell density for normalization of absorbance values to cell amounts.

Storage is at 4°C with a stated stability of 6 months.