TIMP4 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit provides qualitative detection of TIMP4 (tissue inhibitor of metalloproteinase 4) protein expression in cultured cells. The assay uses an indirect ELISA format in which TIMP4-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. It is described as a convenient, lysate-free, high-throughput and sensitive tool for determining the relative expression profile of TIMP4 in cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on TIMP4 levels.

The kit is reactive with human, mouse and rat samples and uses a colorimetric detection method read at 450 nm. The dynamic range is greater than 5000 cells. To support data normalization, the kit includes a monoclonal antibody specific for human GAPDH as an internal positive control, together with a Crystal Violet whole-cell staining step to assess cell density. The assay is designed for adherent cell samples and the kit components are stored at 4°C with a stated stability of 6 months.

TIMP-4 is a key regulator of extracellular matrix remodeling and has been implicated in various diseases, including cancer, arthritis and cardiovascular disorders. The protein functions as a metalloproteinase inhibitor, acting on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9 by binding to their catalytic zinc cofactor.