TIF-IA Colorimetric Cell-Based ELISA Kit

A cell-based colorimetric ELISA from Assay Genie enables quantitative analysis of TIF-IA levels in cell lysates and tissue extracts. The assay uses an indirect ELISA format in which TIF-IA is captured by specific primary antibodies and detected via HRP-conjugated secondary antibodies, producing a colorimetric signal measured at 450 nm. It supports high-throughput screening of treatments, inhibitors such as siRNA or chemicals, and activators on TIF-IA expression in cultured cells without the need for lysate generation.

Normalisation is achieved using an included anti-GAPDH monoclonal antibody as an internal positive control, together with an optional Crystal Violet whole-cell staining step to account for differences in cell density. The assay has a dynamic range of > 5000 Cells, is reactive with human samples, and is provided in a 96-well microplate format suitable for high-throughput workflows on adherent cells. Storage stability is 4 °C for 6 months. Research area: Epigenetics and Nuclear Signaling.

TIF-IA, also known by the gene symbol RRN3, is an RNA polymerase I-specific transcription initiation factor with a molecular weight of 53,315 Da. It acts as a growth-dependent regulator of ribosomal RNA synthesis and is implicated in ribosome biogenesis and protein synthesis.