Thyroid Hormone Receptor beta Transcription Factor Activity Assay

Assay Genie's DNA-Binding ELISA Kit quantifies the functional DNA-binding activity of Thyroid Hormone Receptor beta (TRβ, NR1A2) in nuclear and whole-cell lysates. Each microplate provides 96 Assays and uses an absorbance-based readout. The kit is validated for Human, Mouse and Rat samples, supporting research on thyroid hormone (T3/T4) signaling, metabolic gene regulation, and TRβ mutations linked to hepatocellular carcinoma and resistance to thyroid hormone (RTHβ). Reagents should be stored at 4°C, with the standard kept frozen at -20°C in single-use aliquots to limit freeze-thaw cycles. Typical workflow involves preparing nuclear extract from cultured cells or tissue, incubating lysate with an immobilized TRβ response element (TRE) probe, and detecting bound receptor with a specific antibody.