Thyroid Hormone Receptor alpha Transcription Factor Activity Assay

Profile Thyroid Hormone Receptor alpha (TRα) DNA-binding activity with this transcription factor ELISA from Assay Genie, which captures only the receptor fraction actively engaging its thyroid hormone response element (TRE) consensus sequence rather than total TRα protein levels. Nuclear or whole-cell lysates are added to a TRE-coated plate; receptor-bound wells are then detected with a TRα-specific antibody in a colorimetric readout.

The assay is configured as a 96 Assays microplate kit and accepts nuclear and cell lysates from human, mouse, and rat samples, supporting cross-species comparison of TRα-mediated transcriptional regulation. Routine applications include screening small molecules or hormones that modulate thyroid hormone signaling, time-course studies of TRα activation following ligand stimulation, and profiling receptor activity in nuclear extracts from treated cell lines or tissue biopsies.

Each kit supplies the pre-coated plate, detection antibody, and reagents sufficient to run the full plate. The product is for research use only and is intended for laboratory studies of nuclear receptor biology, endocrinology, and gene regulatory mechanisms. Store unused components refrigerated between runs and consult the enclosed insert for detailed handling and buffer preparation steps before first use.