TGF beta3 Colorimetric Cell-Based ELISA Kit

This cell-based ELISA from Assay Genie is designed for the qualitative measurement of TGF-beta3 protein expression in cultured cells. It uses an indirect ELISA format in which TGF-beta3 is captured by specific primary antibodies and detected with HRP-conjugated secondary antibodies, producing a colorimetric reaction read at 450 nm. The kit is supplied in a 96-well microplate format and is described as a sensitive, high-throughput, lysate-free assay for measuring relative TGF-beta3 levels, including screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators.

Reactivity is reported for human, mouse, and rat samples, with a dynamic range of > 5000 cells and adherent cells as the recommended sample type. For data normalization, the kit includes a monoclonal anti-GAPDH antibody to serve as an internal positive control, plus a Crystal Violet whole-cell staining step to determine cell density so that plating differences can be adjusted. Storage is at 4°C with a stability of 6 months.

TGF-beta3 is a secreted cytokine of the TGF-beta family involved in embryogenesis and cell differentiation, with described roles in tissue repair, embryonic development, and immune regulation. Monitoring its levels can provide insight into the pathogenesis of diseases such as cancer, fibrosis, and autoimmune disorders, supporting studies of cellular signalling pathways and potential therapeutic targets.