TERF1 (Phospho-Ser219)Colorimetric Cell-Based ELISA Kit

A Cell-Based ELISA Kit from Assay Genie enables detection of phosphorylation of TERF1 (Telomeric Repeat Binding Factor 1) at serine 219 in cultured cells. The assay uses a colorimetric detection method with absorbance read at 450 nm and is reactive with human and mouse samples.

Operating as a lysate-free, high-throughput, indirect ELISA, the kit captures Phospho-Ser219 TERF1 using target-specific primary antibodies. HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, supporting qualitative measurement of phosphorylated TERF1. A monoclonal antibody against human GAPDH is included as an internal positive control, and a Crystal Violet whole-cell staining step allows normalization of absorbance values to cell density. The assay is suitable for adherent cells and can be used to evaluate the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on TERF1 phosphorylation.

With a dynamic range of > 5000 Cells, the kit supports research into telomere maintenance, cellular senescence, and genome stability, with relevance to aging, cancer, and other age-related diseases. The sub-type is Phospho and the research area is Cell Cycle.