Telomeric Repeat Binding Factor 1 Transcription Factor Activity Assay

Quantify functional TRF1 DNA-binding activity with this DNA-binding ELISA Kit from Assay Genie. Telomeric Repeat Binding Factor 1 (TERF1/TRF1) is a core shelterin complex protein that recognizes double-stranded TTAGGG telomeric repeats and regulates telomere length homeostasis. Rather than measuring total TRF1 protein abundance, the assay specifically reports the protein's transcription factor binding activity, delivering mechanistic insight into telomere maintenance, replicative senescence, and DNA damage response pathways.

The kit is validated for use with nuclear and cell lysates prepared from human, mouse, or rat samples, enabling chromatin biology, shelterin complex assembly, and aging research workflows. Reagents are supplied in a vial-based kit format for individual reaction setup, with a microplate-based protocol for plate reader analysis. Researchers investigating telomere dysfunction, shelterin regulation, or TRF1-mediated control of telomere length in normal and disease contexts will find this assay a useful functional tool.