TCOF1 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA kit provides a qualitative read-out of TCOF1 (Treacle Ribosome Biogenesis Factor 1) protein expression directly in cultured cells. The assay uses an indirect ELISA format: TCOF1 is captured by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies, producing a colorimetric signal measured at 450 nm. Described by the manufacturer as lysate-free, high throughput and sensitive, the kit can be used to measure relative TCOF1 levels in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on TCOF1 expression.

Reactivity is reported for human and mouse samples, with adherent cells as the specified sample type. The stated dynamic range is > 5000 Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control to normalise target absorbance, and Crystal Violet whole-cell staining can be used to determine cell density and adjust for plating variation. The kit is supplied in a 96-well microplate format and, according to the manufacturer, is stable for 6 months when stored at 4 °C.

TCOF1 (treacle) is a nucleolar protein implicated in nucleolar-cytoplasmic transport, early embryonic development of the craniofacial complex, and certain stages of ribosome biogenesis. Defects in TCOF1 are associated with Treacher Collins syndrome type 1 (TCS1), a disorder of craniofacial development.