TCF7 Transcription Factor Activity Assay

The TCF7 Transcription Factor Activity Assay from Assay Genie is a DNA-binding ELISA kit that quantifies the functional DNA-binding activity of TCF7 (T-cell factor 1), a key nuclear effector of the canonical Wnt/β-catenin signaling cascade. The 96 Assays format plate is pre-coated with a double-stranded oligonucleotide containing the consensus TCF/LEF response element, which captures active TCF7 protein from prepared nuclear and cell lysates of human, mouse, or rat origin. A TCF7-specific detection antibody then binds the captured factor, producing an absorbance signal that reflects transcriptional activation status.

This kit supports research into Wnt-driven T-cell lineage commitment, memory CD8+ T cell differentiation, thymocyte development, and oncogenic contexts where β-catenin signaling is dysregulated. The microplate-based workflow accommodates parallel analysis of multiple treatment conditions, with internal positive and negative controls provided to validate binding specificity. Reagents should be stored refrigerated to maintain antibody stability, and nuclear extracts are best prepared fresh or aliquoted to limit freeze-thaw degradation. Intended for research use only.