TBX18 Transcription Factor Activity Assay

The TBX18 Transcription Factor Activity Assay from Assay Genie quantifies the DNA-binding activity of TBX18 (T-box transcription factor 18) in nuclear and whole-cell lysates. TBX18 plays important roles during embryogenesis, including ureteric bud induction, renal mesenchyme specification, and the development of head and neck musculature, and it has been used as a reprogramming factor to convert fibroblasts into sinoatrial node-like pacemaker cells. The kit provides a microplate pre-coated with a double-stranded oligonucleotide containing the TBX18 consensus binding site; active TBX18 in your lysate binds the immobilized probe and is detected with a specific primary antibody followed by an absorbance-based readout. Researchers studying CAKUT, cardiac lineage specification, or developmental gene regulatory networks can use this assay to monitor TBX18 activity across human, mouse, and rat samples. Components should be stored refrigerated at 4°C; for long-term storage, freeze at -20°C and protect reagents from light. Avoid repeated freeze-thaw cycles of the working buffers. The kit contains reagents for 96 Assays and is for research use only.