TBX10 Colorimetric Cell-Based ELISA

A Cell-Based ELISA Kit from Assay Genie is designed for the quantitative measurement of TBX10 in cell lysates and culture media. Using a colorimetric readout measured at 450 nm, the assay provides a high-throughput, sensitive, lysate-free method to detect TBX10 protein expression profiles in cultured cells and to screen the effects of treatments, inhibitors, or activators on TBX10.

The kit employs an indirect ELISA format in which TBX10 is captured by TBX10-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction on substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalisation, and a Crystal Violet whole-cell staining step can be used to determine cell density. The assay is configured for adherent cells, with a stated dynamic range of > 5000 Cells, and reagents are rated for storage at 4°C with a stability of 6 Months. The listed research area is Epigenetics and Nuclear Signaling, and the target, TBX10 (T-box transcription factor TBX10, UniProt O75333), is a T-box family transcription factor involved in developmental processes, including cardiac development and function, with dysregulation linked to heart and lung disorders.