TBC1D3 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA kit is designed for adherent human cells and provides a lysate-free, high-throughput assay for measuring TBC1D3 protein expression in cultured cells. The qualitative, indirect ELISA format uses TBC1D3-specific primary antibodies with HRP-conjugated secondary antibodies, producing a colorimetric readout at 450 nm. It can be used to measure relative TBC1D3 levels in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on TBC1D3 expression.

Because the assay is qualitative, two normalisation approaches are included: a monoclonal anti-human GAPDH antibody serves as an internal positive control, and Crystal Violet whole-cell staining may be used to determine cell density. The dynamic range is > 5000 Cells, the format is a 96-well microplate, and storage stability is 4 °C for 6 Months.

TBC1D3 (UniProt Q8IZP1) is reported to act as a GTPase-activating protein for RAB5, with no activity on RAB4 or RAB11, and is classified as an oncoprotein. The human ortholog maps to chromosomal location 17q12, and the protein has a molecular weight of 62,168 Da.