TAF5L Colorimetric Cell-Based ELISA

A cell-based ELISA kit from Assay Genie designed for qualitative measurement of TAF5L protein expression in cultured human cells. The kit employs an indirect ELISA format in which TAF5L is captured by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction measured at 450 nm. It is intended for analysing relative TAF5L levels in cell lysates and supernatants, as well as for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on TAF5L expression.

The kit is supplied in a 96-well microplate format and is suited for use with adherent cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, while a Crystal Violet whole-cell staining step may be used to determine cell density and adjust for plating differences. The stated dynamic range is greater than 5000 cells, and the kit is stored at 4 °C with a stability of 6 months. It falls within the Epigenetics and Nuclear Signaling research area.

TAF5L functions as a component of the PCAF histone acetylase complex, which can efficiently acetylate histones in a nucleosomal context and is considered the human counterpart of the yeast SAGA complex. It also acts as a component of the TFIID complex, essential for the initiation of transcription in eukaryotic cells. The protein is classified as a transcription factor, with reported molecular functions including histone acetyltransferase activity, protein binding, transcription coactivator activity, and transcription factor activity. Dysregulation of TAF5L has been implicated in various diseases, including cancer and developmental disorders, making it a target of interest for further research.